This presentation will explore the vital role of specialised tissue culture techniques in safeguarding Australia's threatened botanical heritage. Drawing on current work funded by the Australian Flora Foundation, the talk will detail the practical development and application of tissue culture protocols designed specifically to rescue and propagate the endangered Macadamia jansenii. The session will highlight how advanced plant science methods can be directly applied to overcome propagation bottlenecks and provide real-world solutions for the conservation of vulnerable native species.